少妇人妻精品,综合亚洲婷婷,美国av鲁鲁鲁在线,人妻熟妇精品无码久久久久

技術(shù)文章您的位置:網(wǎng)站首頁 >技術(shù)文章 > 化學(xué)腫瘤基因組學(xué)實驗室使用英克胎牛血清發(fā)表已見刊
化學(xué)腫瘤基因組學(xué)實驗室使用英克胎牛血清發(fā)表已見刊
更新時間:2022-05-24   點擊次數(shù):860次

清華大學(xué)化學(xué)腫瘤基因組學(xué)國家重點實驗室使用Invigentech胎牛血清發(fā)表文章到Cell Reports Medicine已見刊。


一、2020第四季度,清華大學(xué)化學(xué)腫瘤基因組學(xué)國家重點實驗室發(fā)表標題為:An Automated Organoid Platform with Inter-organoid Homogeneity and Inter-patient Heterogeneity的文章到Cell Reports Medicine;

二、使用invigentech(英克)A6903FBS-500,FBS Uruguay Origin胎牛血清對小鼠肺,肝,腎,胰腺,脾臟, 心臟等細胞進行培養(yǎng);

三、文章部分內(nèi)容見下:

Mouse tissue processing

BALB/c mice (male, 6 - 8-weeks old) were euthanized by excessive CO2. Then, the organs (lung, liver, kidney, pancreas, spleen,heart) were isolated and cut into small pieces (1 - 3 mm3 for each piece). After being washed with cold PBS (1 3 ) for three times and supplemented with 2.0% penicillin-streptomycin (vol/vol, GIBCO), the tissues were digested by 1.0 mg/mL collagenase type I (Sigma-Aldrich) and 2.0% fetal bovine serum (FBS) (vol/vol, Invigentech) on an orbital shaker at 37C and incubated for 1 - 2 h. After digestion, the tissues were sheared by 10 mL plastic pipettes. The suspension was passed through a 100 mm filter (Falcon), and centrifuged at 1000 rpm for 5 min. The pellet was resuspended in 5 mL red blood cell lysis buffer (Roche) for 5 min at room temperature. Afterward, the suspension was recentrifuged at 1000 rpm for 5 min, and the pellet was resuspended in DMEM/F12 medium.The cell number was counted for organoid fabrication.



©2025  上海創(chuàng)凌生物科技有限公司 All Rights Reserved.  網(wǎng)站地圖  滬ICP備2023009255號-1  管理登陸  技術(shù)支持:化工儀器網(wǎng)
在線客服 二維碼

掃一掃,關(guān)注我們

午夜久久伦理| 丁香九九九九九九九| 爆乳女教师一区二区三区| 欧美艹艹艹久久久| 激情中文字幕一区二区| 加勒比免费资源一区二区的,| 男女日B色视屏| 国产婷婷色五月| 亚洲欧美天天视频| 97干视频网站| AV女优一区二区三区在线观看| 大奶网一区| 欧美激情一区二区不卡蜜桃| 精品丝袜国产高清自在线拍| 夫妻毛片免费观看网| 91av酒店| 亚洲综合一区二区三区免费| 欧美,日韩,国产,一级| 曰本免费在线视频| 一区二区不卡看欧美| 国产a精品| 国产在线观看大鸡巴| www.wuyefuli| 亚洲乱色熟女三区| 久久久久青青草| 国产97亚洲| 欧美图片小说视频区综合| 青青在线2019视频| 亚洲精品乱码8久久久久久日本| 色欲国产日韩一区二区在线| 日本女优东京一区二二区| 熟女乱91| 日韩靠B视频| 色婷婷91人久久久| 欧美黄片免费视频在线| 日一本一人日B视频| 国产伦精品一区二区三区二| 日本十八一区二区久一| 欧美成人在线视频观看123| 91精品国语对白人妻刺激| 逼逼,免费看|